Chromatographic Qualification and Quantification of Antibiotic Residue in Poultry Meat

Background and Objective: Antibiotic residues in food-producing animals create a substantial threat to public health. Antibiotic residues in food items are attributable to the misuse of antibiotics in veterinary medications. Earlier research in Bangladesh has found a significant incidence of antibiotic residues in animal-based foods, including chicken tissue. The objective of the study screening of antibiotic residues in broiler and native chicken liver, kidney and breast muscle and to estimate Amoxicillin residues from broiler liver and kidney from local markets in Bangladesh. Materials and Methods: Six months research on antibiotic residue has been conducted to examine the risk to human health. A total of 120 poultry specimens (60 broiler and 60 native chicken) were obtained from several marketplaces around the Chittagong Metropolitan Area for this study, including liver, kidney and breast muscles. Thin layer chromatography (TLC) has been used to test the specimens for Tetracycline, Amoxicillin, Sulfanilamide and Ciprofloxacin accompanied by Ultra-High-Performance Liquid Chromatography (UHPLC) on selected positive tested cases. Results: The TLC results showed that 48.3% of broiler and 23.3% of native chicken samples were positive. In the research, broiler and native chicken liver had the most antibiotic residues respectively 70 and 35%. There was a significant (p<0.05) difference between the Tetracycline, Amoxicillin, Sulfanilamide and Ciprofloxacin residues of native chicken and the Tetracycline, Amoxicillin, Sulfanilamide and Ciprofloxacin residues of broiler chicken. Additionally, Amoxicillin levels were found to be 16.9-149.9 g g^–1 in the liver and 45.3-60.5 g g^–1 in the kidney using UHPLC. The greatest and shortest peak times were 4.7 and 5.1 min, correspondingly. Conclusion: It is concluded that antibiotics are administered to animals without the need for a prescription and the right dose is not maintained due to withdrawal symptoms. For the sake of public health, the research suggests that a withdrawal time be implemented, as well as good surveillance and careful drug usage in animals.